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KMID : 1161420140170070772
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Journal of Medicinal Food
2014 Volume.17 No. 7 p.772 ~ p.781
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Reciprocal Regulation of Adipocyte and Osteoblast Differentiation of Mesenchymal Stem Cells by Eupatorium japonicum Prevents Bone Loss and Adiposity Increase in Osteoporotic Rats
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Kim Min-Ji
Jang Woo-Seok
Lee In-Kyoung
Kim Jong-Keun
Seong Ki-Seung
Seo Cho-Rong
Song No-Joon
Bang Min-Hyuk
Lee Young-Min
Kim Haeng-Ran
Park Ki-Moon
Park Kye-Won
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Abstract
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Pathological increases in adipogenic potential with decreases in osteogenic differentiation occur in osteoporotic bone marrow cells. Previous studies have shown that bioactive materials isolated from natural products can reciprocally regulate adipogenic and osteogenic fates of bone marrow cells. In this study, we showed that Eupatorium japonicum stem extracts (EJE) suppressed lipid accumulation and inhibited the expression of adipocyte markers in multipotent C3H10T1/2 and primary bone marrow cells. Conversely, EJE stimulated alkaline phosphatase activity and induced the expression of osteoblast markers in C3H10T1/2 and primary bone marrow cells. Daily oral administration of 50?mg/kg of EJE for 6 weeks to ovariectomized rats prevented body weight increase and bone mineral density decrease. Finally, activity-guided fractionation led to the identification of coumaric acid and coumaric acid methyl ester as bioactive anti-adipogenic and pro-osteogenic components in EJE. Taken together, our data indicate a promising possibility of E. japonicum as a functional food and as a therapeutic intervention for preventing osteoporosis and bone fractures.
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KEYWORD
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bone marrow, C3H10T1/2, coumaric acid, Eupatorium japonicum, osteoporosis, ovariectomized rat
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